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WebJan 11, 2024 · All the insects were homogenised in liquid nitrogen, and the genomic DNA was extracted using the SpeedTools tissue DNA extraction kit (Biotools, Spain) following the manufacturer's instructions. WebIn bio.tools, we are striving to provide a comprehensive registry of software and databases, facilitating researchers from across the spectrum of biological and biomedical science to find, understand, utilise and cite the resources they need in their day-to-day work. Everything from simple command-line tools and online services, through to ... grace and taylor events
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WebNov 1, 2024 · In both assays, final reaction volume was 50 μl containing 1X buffer (Biotools ®, Spain), 1.5 mM MgCl 2 (Biotools ®, Spain), 1U HotSplit DNA Polymerase (Biotools ®, Spain), 200 μm dNTPs mixture (Biotools ®, Spain), 25 pmol of each primer, 2 μl of BSA (20 mg/ml, Roche ®, Switzerland). WebIn all cases the total amount of cells used by PCR reaction was 60.000. M: DNA marker (Biotools, Spain) A further step for the applicability of the method was the use of directly bloom biomass as DNA template. To challenge our … WebJan 29, 2024 · The reaction mix consisted of 1X Quantimix (Biotools, Spain), 0.24 μM of each primer, 0.12 μM of TaqMan probe and 3 μl of the template (purified DNA or direct extraction from the spot) in a final volume of 12 μl. The real-time PCR amplification protocol included the following steps: 95 °C for 20 s followed by 45 cycles of 95 °C for 1 s ... grace and taylor coffee