http://sekelsky.bio.unc.edu/lab/In-Fusion.pdf WebClontech Laboratories, Inc. www.clontech.com Protocol No. PT3269-1 2 Version No. PR782357 SMART™ RACE cDNA Amplification Kit User Manual Table of Contents I. …
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WebJun 12, 2013 · Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Avenue, Mountain View, CA 94043, USA U.S. Technical Support: [email protected] United States/Canada 800.662.2566 WebThe tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes in eukaryotic cells in diverse settings, varying from basic … swan street coaches bromsgrove
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WebClontech Laboratories, Inc. www.clontech.com Protocol No. PT3269-1 2 Version No. PR782357 SMART™ RACE cDNA Amplification Kit User Manual Table of Contents I. Introduction & Protocol Overview 4 II. List of Components 9 III. Additional Materials Required 10 IV. General Considerations for SMART RACE Amplification 11 V. Primer Design 12 VI. WebSep 5, 2013 · The inserts were amplified by PCR and cloned into pRetroX-TRE3G (Clontech, 631188). YFP-PARK2 and PINK1-YFP were cloned in pBMN-LacZ (Allele Biotechnology, ABP-PVL-10011; LacZ was removed) and pRetroX-IRES-DsRedExpress (Clontech, 632521), respectively. For transient expression of OTC constructs, cells were … WebSupplier: Clontech Labs 3P 634858 The SMARTer RACE 5'/3' Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART (Switching Mechanism At 5' End of RNA Template) technology, and facilitates the performance of 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) PCR with the kit's Universal Primer Mix. ... swan street car park