Strain bj3505

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Web1 Sep 2016 · To determine the localization of Vtc5, the protein was genomically tagged with yeGFP at its C terminus in protease-deficient strains (BJ3505) and protease-proficient strains (DKY6281). Vtc5-yeGFP yielded a relatively weak signal around the vacuole rim in BJ3505 cells. No fluorescence signal was detected in other parts of the cell. WebVacuoles were isolated from yeast strains BJ3505 and DKY6281 as described (30). Large-scale frozen vacuole preparations of BJ3505 vacuoles were used for studies of …

Excess vacuolar SNAREs drive lysis and Rab bypass …

Web1 Sep 1999 · One strain (BJ3505) has a deletion in the gene encoding the major vacuolar proteases and therefore accumulates catalytically inactive pro-alkaline phosphatase. The … chinese pain relief methods

Role of the Vtc proteins in V-ATPase stability and …

WebThe protein Ixr1p fused to the FLAG ™ peptide at the carboxyl-terminal end was expressed in the yeast strain BJ3505 . Cells were grown at 30 °C beyond 48 h in YPHSM medium (8% Bacto peptone, 1% yeast extract, 3% glycerol and 1% dextrose) until OD 600 nm 6.0, coinciding with the maximum expression. Cells were lysed in TBS buffer (50 mM Tris ... Web1 Oct 2010 · BJ3505 CBP-Vam3p nyv1Δ was used for trans-SNARE complex isolation (Collins and Wickner, 2007). NHX1 was deleted from BJ3505, DKY6281 and BJ3505 CBP … Web1 Aug 2000 · Vacuole Isolation. Vacuoles were isolated from yeast strains BJ3505 and DKY6281 as described ().Large-scale frozen vacuole preparations of BJ3505 vacuoles were used for studies of glutathione S-transferase (GST)-Ypt7p association and for immunoprecipitation experiments. Spheroplast pellets were resuspended in an equal … chinese padstow

A Ypt/Rab effector complex containing the Sec1 homolog Vps33p …

Na+/H+ exchanger Nhx1p regulates the initiation of …

Web1 Dec 2003 · Batch fermentations were carried out to examine and compare the patterns of cell growth and sugar metabolism for the two recombinant S. cerevisiae BJ3505 strains, δXR and δXR/STH. As shown in Fig. 1, the expression of the STH gene did not make significant changes in glucose utilization, cell growth, ethanol accumulation, and xylitol … WebDownload scientific diagram Monitoring of variants MEL1 expressed in strain BJ3505 using YPHSM culture medium. Extracellular (clear grey), intracellular (dark grey) activity (a), and … grand ridge mountain bike trailWebacidocalcisome phosphate homeostasis Introduction Phosphate is a limiting factor for the growth of living organisms. It is mainly taken up by the cells as P i and incorporated in biological molecules, including ATP, nucleic acids, and phospholipids. grand ridge ptsa

"Web8 May 2013 · Strains . BJ3505 and DKY6281 were used for fusion assays (Table 1) ().YCF1 was deleted from BJ3505 and DKY6281 by homologous recombination with the kanMX6 cassette using PCR products amplified from pFA6a-kanMX6 with homology flanking the YCF1 coding sequence. The PCR product was transformed into BJ3505 and DKY6281 by … " - Strain bj3505

Strain bj3505

Ixr1p regulates oxygen-dependent HEM13 transcription

Web1 Nov 2001 · The strains BJ3505 VPS33::ProtA and BJ3505 VAM3ΔN VPS33::ProtA were created by transformation of a PCR fragment containing the sequence encoding Protein A … Weba Monitoring of the extracellular α-galactosidase activity of cultures with the recombinant strains BJ3505/YEpMEL1 (squares), BJ3505/ YEpMEL1Flag (circles) and BJ3505/YEpMEL1His...

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Web1 Nov 2001 · The strains BJ3505 VPS33::ProtA and BJ3505 VAM3ΔN VPS33::ProtA were created by transformation of a PCR fragment containing the sequence encoding Protein A and a kanMX6 selection marker with flanking regions of the VPS33 3′-region and the downstream sequence of the terminator ( Knop et al., 1999 ). WebThe strain BJ3505 carries the wild-type PHO8 gene but lacks genes encoding the major vacuolar proteases that activate the Pho8p phosphatase. Thus, vacuoles isolated from …

WebThe Saccharomyces Genome Database (SGD) provides comprehensive integrated biological information for the budding yeast Saccharomyces cerevisiae along with search and analysis tools to explore these data, enabling the discovery of functional relationships between sequence and gene products in fungi and higher organisms. Explore SGD. WebThe coding sequence of truncate gp160 was amplified by polymerase chain reaction (PCR) and cloned into yeast expression vector YEpFLAG-1 to construct plasmid o

Web15 Mar 2003 · The plasmid was transformed into yeast strains BJ3505 and DKY6281 using the URA3 marker. For the construction of Vtc3-GFP, GFP was chromosomally integrated … WebBJ3505 cells were grown in YEPD to stationary phase, and then suspended in 340 ~1 of 0.1 M sodium phosphate buffer (pH 7.0) and treated with 10 ~1 EMS for 60 min at 30°C; the survival rate was ... teinase-deficient strain, BJ3505, was mutagenized and cells that did not accumulate AB’s in the vacuoles under ...

Web27 Jul 2015 · In brief, strain BJ3505 was grown to mid-log phase in 4 liters of YPD medium. Cells were harvested, washed once with PS buffer containing 150 mM KOAc and 5 mM MgOAc, resuspended in the same buffer containing 1 mM PMSF and 10 µM leupeptin, and quick-frozen by drop-wise addition to liquid nitrogen. The frozen cells were mixed with …

WebUsing different Rhizopus sp. strains as fermentation agents, this paper describes a global strategy to identify interactions between cultivation parameters (pH 4.75–7.75, 7.5–82.5 g/l glucose ... grand ridge middle school floridaWebstrains DKY6281 and BJ3505. The strain DKY6281 contains normal vacuolar proteases but lacks the major vacuolar phos-phatase encoded by the PHO8 gene. The strain BJ3505 … chinese painted button quailWeb10 Oct 2024 · The aim of this work has been to optimize the recombinant production and further characterization of α-galactosidase of Saccharomyces cerevisiae. Results: The … chinese painted bottles insideWebSeasonal and developmental variations in the transcriptional activities induced by Maclura pomifera and Morus rubra extracts in S . cere 6 isiae strain BJ3505. (A) Transcriptional activity... chinese painting classes near meWeb1 Dec 2012 · BJ3505 CBP (calmodulin-binding peptide)–Vam3 nyv1Δ was used for trans-SNARE complex isolation (Table 1). YBT1 was deleted from BJ3505, DKY6281 and … grand ridge orthodontics grand rapids miWeb13 Oct 2024 · Trans-SNARE pairing was analyzed with vacuoles prepared from DKY6281 vam3Δ, BJ3505 nyv1Δ, and BJ3505 nyv1Δ VAM3ΔN. Vacuoles (120 μg each) were mixed … grand ridge ptsa issaquahWeb1 Dec 2001 · Vacuolar morphology is unaltered in the Vam3N mutant. Wild-type or mutant BJ3505 strains were incubated with 10 M of the dye FM4-64 (Molecular Probes, Eugene, OR) for 20 min at 30°C in YPD. chinese painting a national fine art of china